Effects of Pretreatment with Pentoxyfilline and N-acetylcysteine on Renal Injury Following Induction of Liver Ischemia-Reperfusion

Authors

  • B Seifi Assistant Professor of Physiology, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • F Delavari M.Sc. Student of Physiology, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • H SHahidi Laboratory Staff Member, Children Medical Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • M Kadkhodaei Professor of Physiology, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • S Mikaeili M.Sc. of Physiology, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • S SHams Associate Professor of Pathology, Children Medical Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract:

Background & Aims: Liver ischemia-reperfusion (IR) is one of the common consequences of liver surgery, especially during liver transplantation which results in organ dysfunction. Acute hepatic injury causes systematic inflammatory responses which may finally lead to functional disturbances in remote organs such as heart, lungs and kidneys. In this study, the effects of a potent inhibitor of inflammatory cytokines (pentoxyfilline, PTX) and a well known antioxidant, (N-acetylcysteine, NAC), was evaluated on renal functional damage and oxidative stress following liver IR. Method: Five groups of six male rats were used. Group one was sham operated. In group 2, 90 min liver partial ischemia was conducted by a clamp around hepatic artery and portal vein and followed by 4 hours of reperfusion. In group 3 and 4, PTX or NAC was injected intraperitoneally before the ischemia, while in group 5 both drugs were co-administered. The levels of ALT, AST, ALP, BUN and creatinine in serum as well as MDA and GSH levels in renal tissues were measured. Results: Significant increase in the serum levels of ALT, AST in IR group is indicative of liver functional damages comparing to sham operated rats. Elevated BUN levels and increased renal tissue MDA and decreased GSH levels in IR group demonstrates a significant kidney functional damage and oxidative stress comparing to sham group. Administration of PTX alone and PTX+NAC prevented the IR-induced increase in renal MDA levels. Administration of both drugs and their co-administration prevented the reduction in renal GSH levels. Conclusion: Pretreatment with PTX and NAC before liver IR induction may prevent renal oxidative stress by protection of cellular GSH concentration and a reduction in MDA levels.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Pretreatment with pentoxifylline and N-acetylcysteine in liver ischemia reperfusion-induced renal injury.

BACKGROUND AND AIMS Acute hepatic injury causes systematic inflammatory responses which may finally lead to functional disturbances in remote organs. In this study, the effects of an inhibitor of inflammatory cytokines (pentoxifylline, PTX) and a well-known antioxidant, N-acetylcysteine (NAC), were evaluated on renal damage and oxidative stress following liver ischemia reperfusion (IR). METHO...

full text

Effects of N-acetylcysteine on liver remote injury after skeletal muscle ischemia reperfusion in rats.

BACKGROUND/AIMS This study evaluated the effects of N-acetylcysteine as a scavenger of radical oxygen species on liver injury as a remote organ after skeletal muscle ischemia reperfusion. MATERIALS AND METHODS Twenty male Wistar rats were allocated randomly into two experimental groups: ischemia reperfusion (I/R) and ischemia reperfusion + N-acetylcysteine (I/R+NAC). All animals were undergon...

full text

Pretreatment effects of metformin on behavioral manifestations of global ischemia/reperfusion injury in rats

Introduction: Metformin (MET) as an oral antidiabetic drug has pleiotropic effects such as antioxidative and neuroprotective properties. In the current study we aimed to investigate the probable effects of Pretreatment MET therapy in global ischemia-reperfusion in rats. Materials and Methods: In this study, 40 male Wistar rats (200-220 g) were used. Animals were randomly divided into four g...

full text

Renoprotective effects of GABA on ischemia/reperfusion- induced renal injury in hyperglycemic male and female rats

Introduction: Acute kidney injury (AKI) has been known as a complex clinical complication in diabetic patients. The main cause of AKI is ischemia/reperfusion injury (IRI). This study was designed to investigate the protective effects of GABA on renal IRI in hyperglycemic female and male rats. Methods: Sixty STZ induced diabetic male and female Wistar rats were categorized in 10 groups (5 fem...

full text

Effects of liver ischemia-reperfusion on renal functional and oxidative stress indices

Introduction: Liver ischemia/reperfusion (IR) is a major clinical problem, which occurs during several conditions such as liver damage, trauma and transplantation. Recent studies indicate that IR-induced acute liver failure causes injuries of distant organs such as heart and lungs by systematic inflammatory responses. Therefore, in the present study, effects of hepatic IR induction were stud...

full text

berberine improves liver injury following renal ischemia reperfusion in rats

this study investigated the effect of berberine on the hepatic dysfunction and histological damage induced by renal ischaemia/ reperfusion (i/r) at an early stage. there were four groups (n=7). in ber+i/r group, rats received berberine (ber; 15 mg/kg/day) orally for 7 days before induction of ischemia. i/r group received distilled water orally for 7 days. in sham and ber+sham groups in which ar...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 17  issue 1

pages  1- 9

publication date 2010-12-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023